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RNA Interference in Mammalia Cells by RNA-3’-PNA Chimeras

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Author(s): Nicoletta Potenza | Loredana Moggio | Giovanna Milano | Vincenzo Salvatore | Benedetto Di Blasio | Aniello Russo | Anna Messere

Journal: International Journal of Molecular Sciences
ISSN 1422-0067

Volume: 9;
Issue: 3;
Start page: 299;
Date: 2008;
Original page

Keywords: modified siRNA | RNA interference | RNA-PNA chimeras | Peptide Nucleic Acids | RNA.

ABSTRACT
The discovery of siRNAs as the mediators of RNA interference has led to an increasing interest in their therapeutic applications. Chemical modifications are introduced into siRNAs to optimize the potency, the stability and the pharmacokinetic properties in vivo. Here, we synthesize and test the effects of RNA-3’-PNA chimeras on siRNA functioning and stability. We demonstrate that the chemical modifications are compatible with the siRNA machinery, because all the PNA-modified siRNAs can efficiently mediate specific gene silencing in mammalian cells. Furthermore, we find that the modification on the sense strand of siRNA results in an increased persistence of the activity, whereas modification on both strands results in enhanced nuclease resistance in serum.
Affiliate Program     

Tango Jona
Tangokurs Rapperswil-Jona