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A role for non-coding Tsix transcription in partitioning chromatin domains within the mouse X-inactivation centre

Author(s): Navarro Pablo | Chantalat Sophie | Foglio Mario | Chureau Corinne | Vigneau S├ębastien | Clerc Philippe | Avner Philip | Rougeulle Claire

Journal: Epigenetics & Chromatin
ISSN 1756-8935

Volume: 2;
Issue: 1;
Start page: 8;
Date: 2009;
Original page

Abstract Background Delimiting distinct chromatin domains is essential for temporal and spatial regulation of gene expression. Within the X-inactivation centre region (Xic), the Xist locus, which triggers X-inactivation, is juxtaposed to a large domain of H3K27 trimethylation (H3K27me3). Results We describe here that developmentally regulated transcription of Tsix, a crucial non-coding antisense to Xist, is required to block the spreading of the H3K27me3 domain to the adjacent H3K4me2-rich Xist region. Analyses of a series of distinct Tsix mutations suggest that the underlying mechanism involves the RNA Polymerase II accumulating at the Tsix 3'-end. Furthermore, we report additional unexpected long-range effects of Tsix on the distal sub-region of the Xic, involved in Xic-Xic trans-interactions. Conclusion These data point toward a role for transcription of non-coding RNAs as a developmental strategy for the establishment of functionally distinct domains within the mammalian genome.

Tango Jona
Tangokurs Rapperswil-Jona

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