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Screening and Identification of a Quercetin-Producing Bacterium Paenibacillus glucanolyticu D3

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Author(s): Zhitang Lu | Yating Zhang | Nan Shi

Journal: Environment and Natural Resources Research
ISSN 1927-0488

Volume: 2;
Issue: 1;
Date: 2012;
Original page

ABSTRACT
Quercetin is a kind of flavonoid, which has outstanding drug action. To develop the production technology of quercetin by microorganism, strains that can transform rutin to quercetin were isolated from soil, screened by TLC and re-screened by HPLC. As a result, strain D3 with the highest transformation ability was obtained. The hydrolyze enzyme was intracellular enzyme and transformation rate was 41.6% within a system comprising of 50mg wet cells in 4mL of 0.1% rutin solution and transformed for 72hr at 25°C. Strain D3 was identified as Paenibacillus glucanolyticus by integrating the 16S rDNA phylogeny analysis result and a variety of morphological, physiological and biochemical features.
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