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Screening of Potential Strains and Co-substrate for Production of Cellulase Enzyme using Sewage Treatment Plant Sludge as Major Substrate

Author(s): Afifah Drani | Md. Zahangir Alam | Suleyman A. Muyibi

Journal: Biotechnology
ISSN 1682-296X

Volume: 10;
Issue: 2;
Start page: 197;
Date: 2011;
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Keywords: filamentous fungi | media optimization | bioconversion | Endo-glucanase | sewage treatment plant sludge

Potential fungal strains belonging to the genera of Penicillium, Trichoderma and Aspergillus were isolated from various waste sources and tested for their capability to produce cellulase enzyme using Sewage Treatment Plant (STP) sludge as a major substrate. The isolation technique was used in the Potato Dextrose Agar (PDA) media incorporated with the sewage sludge (0.5% Total Suspended Solids, TSS). In addition 1 mL of streptomycin was used to control the growth of bacteria. Colonies appeared in the plate were transferred to the fresh PDA plate for purification and identification. Out of 35 strains, five strains were identified as the potential strains to produce cellulase through Filter Paper Activity (FPA) assay. These five promising strains were TH(U), S-105A and P2-STP isolated from STP sludge, P1-EFB isolated from Empty Fruit Bunched (EFB) compost and O-102A isolated from rotten orange. Various carbon sources; wheat flour, cassava flour, commercial sugar and cellulose were evaluated as a co-substrate with and without EFB supplement using these five potential strains. The strain O-102A was identified as the most promising strain that able to produce cellulase enzyme using STP sludge as a major substrate, cellulose as a co-substrate with supplement of oil palm Empty Fruit Bunches (EFB).
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