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Segregation Analysis of p53, c-myc and DNA Ploidy Using Flow Cytometry Among Egyptian Families with Childhood Leukemia

Author(s): Mohammad S. Al-Haggar | Ahmad A. Settin | Mohamed Ragab | May Al-Shahat | Hisham Abdel-Hadi | El-Shahat Toson | Mona A. Samy | Rabab Abu Al-Kasem | Soheir Yahya | Zakaria Al-Morsy

Journal: Journal of Medical Sciences
ISSN 1682-4474

Volume: 6;
Issue: 6;
Start page: 906;
Date: 2006;
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Keywords: Familial risk | childhood leukemia | flow cytometry | p53 | c-myc | ploidy pattern

The present study aimed to check for segregation of some oncogenic markers (p53, c-myc) and DNA ploidy pattern in Egyptian families of children with acute lymphoblastic leukemia (ALL) to determine whether there is an actual risk for cancer among these families. This case-controlled study included 20 Egyptian children with ALL, their median age 6.5 years (interquartiles 2.1-12.5) with males/females 16/4. They were enrolled at presentation to Haematology-Oncology Unit of Mansoura University Children`s Hospital, Egypt. The study included also their first-degree relatives (20 fathers, 20 mothers, 44 healthy sibs) and control group; 20 healthy subjects with median age 6.9 years (interquartiles 3.2-13.1) and sure negative family history of cancer. Blood sampling was done for all persons followed by cell isolation for Flow Cytometric analysis (FCM) of DNA ploidy, apoptosis and other cell cycle parameters. p53 and c-myc protein expression were also assessed by FCM using monoclonal-antibody staining technique. p53 and c-myc showed significantly high values in cases compared to controls and relatives (p

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