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Selective Expansion of CD34+ Cells from Mouse Bone Marrow Cultured on LH/P MP-Coated Plates with Adequate Cytokines

Author(s): Satoko Kishimoto | Masayuki Ishihara | Yasuhiro Kanatani | Masaki Nambu | Megumi Takikawa | Yuki Sumi | Shingo Nakamura | Yasutaka Mori | Hidemi Hattori | Yoshihiro Tanaka | Toshinori Sato

Journal: Journal of Tissue Engineering
ISSN 2041-7314

Volume: 2;
Issue: 1;
Date: 2011;
Original page

Keywords: CD34-positive hematopoietic cells (CD34 cells) | cell proliferation | cytokines

Low-molecular-weight heparin/protamine microparticles (LH/P MPs) serve as carriers for controlled release of heparin-binding cytokines. LH/P MPs were stably coated onto plastic surfaces by drying. The purpose of this study is to evaluate a culture method for selective expansion of CD34+ cells using LH/P MPs as cytokine-binding matrix. Ficoll-purified mouse bone marrow cells (mouse FP-BMCs) containing CD34+ cells were cultured on LH/P MP-coated plates in the presence of stem cell factor (SCF), thrombopoietin (Tpo), and Flt-3 ligand (Flt-3) in hematopoietic progenitor growth medium (HPGM) supplemented with 4% heat-inactivated fetal bovine serum (FBS). After 8 days of culture, the total cell count increased 4.6-fold, and flow cytometry analyses revealed that 23.8% of the initial cells and 57.4% of the expanded cells were CD34 positive. Therefore, CD34+ cells were estimated to have increased 11.0-fold. In contrast, cultured CD34+ cells on uncoated tissue culture plates increased 5.8-fold in an identical medium.

Tango Jona
Tangokurs Rapperswil-Jona

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