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The Sequence Analysis of Chinese Scolopendra Subspinipes Mutilans’Stress Transcription Factor p8 cDNA from Poison Gland

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Author(s): HUA Wei-jian | ZOU Yong-mei | CHEN Yu

Journal: Journal of Chongqing Normal University
ISSN 1672-6693

Volume: 28;
Issue: 6;
Start page: 13;
Date: 2011;
Original page

Keywords: Scolopendra Subspinipes Mutilans | poison gland | stress transcription factor | p8/Nuprl/Com1 | homologous evolution | parallel evolution

ABSTRACT
Here we report the complete cDNA sequence of a stress transcription factor p8 from poison gland of Scolopendra Subspinipes Mutilans. The cDNA was 838 bp in full-length, an initiator element TCATTCT was found in its 97 bp 5'-untranslated region, two mRNA rapid degradation signal of ATTTTTA were found in its 525 bp 3'-untranslated region, and one AATAAA was found 13 bp before the poly-A tail. The 216 bp open reading frame(ORF)encodes the p8 nuclear protein of 71 amino acids with the predicted molecular weight of 8.4 kD and the isoelectric point of 9.19. The sequence indentities of the centipede p8 peptide with 10 other p8 expressed in venom and blood-sucking salivary gland of invertebrate were 61%-79% , and the similarities were 80%-91%.There was no PEST rich region near the N term of there invertebrate p8, but the basic helix-loop-helix domain was highly conserved. Phylogenic analysis revealed three striking characteristics in P8 homologous evolution, the grown up of mRNA stability, the decreased peptide stability and also increased major binding site of its bipartite nuclear localization sequences. Analysis on duplicated p8 from 10 vertebrate species suggested the duplication-divergent evolution of p8 gene may start at the time of divergence of bony fish from invertebrate.

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