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A simple RP-HPLC method for quantitation of itopride HCl in tablet dosage form

Author(s): Thiruvengada Rajan V | Mohamed Saleem T | Ramkanth S | Alagusundaram M | Ganaprakash K | Chetty Madhusudhana

Journal: Journal of Young Pharmacists
ISSN 0975-1483

Volume: 2;
Issue: 4;
Start page: 410;
Date: 2010;
Original page

Keywords: Dosage formulation | itopride HCl | method validation | RP-HPLC | UV detection

An isocratic reversed phase high-performance liquid chromatographic method with ultraviolet detection at 220 nm has been developed for the quantification of itopride hydrochloride in tablet dosage form. The quantification was carried out using C 8 column (250 mm × 4.6 mm), 5-μm particle size SS column. The mobile phase comprised of two solvents (Solvent A: buffer 1.4 mL ortho-phosphoric acid adjusted to pH 3.0 with triethyl amine and Solvent B: acetonitrile). The ratio of Solvent A: Solvent B was 75:25 v/v. The flow rate was 1.0 mL -1 with UV detection at 220 nm. The method has been validated and proved to be robust. The calibration curve was linear in the concentration range of 80-120% with coefficient of correlation 0.9995. The percentage recovery for itopride HCl was 100.01%. The proposed method was validated for its selectivity, linearity, accuracy, and precision. The method was found to be suitable for the quality control of itopride HCl in tablet dosage formulation.
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