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Simultaneous determination of ascorbic acid and isoascorbic acid in food by high pressure liquid chromatography

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Author(s): LI Gang

Journal: Shipin Anquan Zhiliang Jiance Xuebao
ISSN 2095-0381

Volume: 4;
Issue: 1;
Start page: 251;
Date: 2013;
Original page

Keywords: ascorbic acid | isoascorbic acid | high pressure liquid chromatography | Venusil HILIC column

ABSTRACT
Objective To establish a high pressure liquid chromatography (HPLC) method for simultaneous determination of the contents of ascorbic acid and isoascorbic acid in food. Methods Ascorbic acid and isoascorbic acid were separated using a Venusil HILIC column (250 mm×4.6 mm, 5 μm, 100 ?), taking acetonitrile-0.1% phosphoric acid solution (90∶10, v/v) as the mobile phase and extractant. Results The average recoveries of ascorbic acid and isoascorbic acid were 96.61% and 96.53%. And their minimum detectable concentration was 0.35 μg/mL and 0.42 μg/mL. Conclusion The method is accurate, sensitive and of precision, and can be used for the determination of ascorbic acid and isoascorbic acid in food.
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