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Simultaneous determination of five marker constituents in Ssanghwa tang by HPLC/DAD

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Author(s): Won Jin | Ma Jin | Um Young | Ma Choong

Journal: Pharmacognosy Magazine
ISSN 0973-1296

Volume: 6;
Issue: 22;
Start page: 111;
Date: 2010;
Original page

Keywords: Herbal medicine | HPLC- DAD | quality control | quantification | Ssanghwa tang

ABSTRACT
A HPLC-DAD method was established for the simultaneous evaluation of five bioactive compounds in Ssanghwa tang (SHT) including glycyrrhizin, paeoniflorin, cinnamic acid, decursin and 6-gingerol. These compounds were separated in less than 40 min using a Dionex C 18 column with a gradient elution system of water and methanol at a flow rate of 1 ml/min. Calibration curve of standard components presented excellent linear regression (R 2 > 0.9903) within the test range. Limit of detection and limit of quantification varied from 0.07 to 0.46 μg/ml and 0.13 to 1.11 μg/ml, respectively. The relative standard deviations (RSDs) of data of the intraday and interday experiments were less than 3.67 and 5.73%, respectively. The accuracy of recovery test ranged from 95.98 to 105.88% with RSD values 0.10- 4.82%.
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