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Simultaneous determination of psuedoephedrine, fexofenadine and loratadine in pharmaceutical products using high resolution RP-HPLC method

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Author(s): Kullai Reddy Ulavapalli1, J. Sriramulu1, Useni Reddy Mallu1 and Varaprasad Bobbarala2*.

Journal: Journal of Pharmacy Research
ISSN 0974-6943

Volume: 4;
Issue: 4;
Start page: 1219;
Date: 2011;
Original page

Keywords: Psuedoephedrine | Fexofenadine | Loratadine | high resolution and RP-HPLC method.

ABSTRACT
High resolution RP-HPLC method has been developed for the simultaneous estimation of Psuedoephedrine, Fexofenadine and oratadine in pharmaceutical products. Separation was achievedby using C18, 150 x 4.6 mm, 5μm column at a temperature of 350C with a gradient mobile phase composed of sol-A: 0.01M pH 3.0 phosphate (KH2PO4) buffer, sol-B: Acetonitrile and gradient program was 0-5min, sol-A: 80-60; 5-10min- sol-A: 60-20; 10-15min- sol-A: 20-20; 5-17min- sol-A: 20-80 and 17-20min- sol-A: 80-80. Flow rate was 0.60 ml per min and measured the absorbance at 210nm. The retention time of Psuedoephedrine, Fexofenadine and Loratadine are 4.3min, 10.4min and 17.0min, respectively. The linearity of the proposed method was evaluated from 5μg per mL to 100μg per mL and the correlation coefficient results were within the limit (not less than 0.999). The developed method has wide applicable for the quantification ofPsuedoephedrine, Fexofenadine and Loratadine in pharmaceutical dosage forms.

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