Academic Journals Database
Disseminating quality controlled scientific knowledge

Simultaneous Determination of Synthetic Phosphodiesterase-5 Inhibitors in Dietary Supplements by Liquid Chromatography-High Resolution/Mass Spectrometry

Author(s): So-Hee Kim | Ho Jun Kim | Junghyun Son | Byoung Wook Jeon | Eun Sook Jeong | Eun Ju Cha | Jaeick Lee

Journal: Mass Spectrometry Letters
ISSN 2233-4203

Volume: 3;
Issue: 2;
Start page: 50;
Date: 2012;
Original page

Keywords: LC-HR/MS | LTQ-Orbitrap | Phosphodiesterase-5 inhibitor | Sildenafil

After success of sildenafil for the treatment of erectile dysfunction, a large number of its analogues have beenapproved from FDA. Recently, the illegal dietary supplements which include sildenafil, vardenafil, tadalafil, or analogues ofthese drugs as ingredient have been widely distributed. Therefore, the determination of the residue of synthetic phosphodiesterase-5 (PDE-5) inhibitors in dietary supplements is highly required due to indiscriminate and unintentional overdose causednausea, chest pains, fainting and irregular heartbeat. In this paper, we report a rapid and sensitive analytical method for thesimultaneous determination of nine phosphodiesterase-5 inhibitors by liquid chromatography-high resolution mass spectrometry.The present method was found to be accurate and reproducible with 40 μg/g of the limit of quantification for the nine PDE-5inhibitors. The developed method can be successfully applied to the analysis of the seven illegal dietary supplements.
Save time & money - Smart Internet Solutions      Why do you need a reservation system?