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Simultaneous estimation of amitriptyline HCl and perphenazine in tablets by UV-Visible spectrophotometric and HPTLC

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Author(s): Dhara J Patel | Vivek Patel

Journal: International Journal of Pharmacy and Biomedical Sciences
ISSN 0976-5263

Volume: 01;
Issue: 02;
Start page: 20;
Date: 2010;
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Keywords: Amitriptyline HCl | Perphenazine | UV/Visible Spectrophotometry | Simultaneous equation method | HPTLC | Densitometric measurement

ABSTRACT
A simple and specific UV/Visible spectrophotometric and HPTLC methods were developed for the simultaneous determination of amitriptyline HCl and perphenazine in tablets. The former is the UV/Visible spectrophotometric method which involves the determination of amitriptyline HCl and perphenazine using the simultaneous equation method at 240.0 and 258.0 nm over the concentration ranges of 2-12 μg/mL for both. The later is the HPTLC method, where the separation of drugs was carried out on Merck thin layer chromatographic aluminium sheets of silica gel 60 F254 using the mobile phase containing carbon tetrachloride: toluene:methanol:triethyl amine (4:3:2:0.2, v/v/v). The separation was followed by densitometric measurements of their spots at 240 nm. The methods were validated for linearity precision, sensitivity, and specificity. The linearity was found to be in the range of 50–600 and 40–480 ng/spot for amitriptyline hydrochloride and perphenazine, respectively. The methods were specific because no chromatographic interferences from the tablet excipients were found and therefore, the proposed methods were successfully applied for the routine quantitative analysis of tablets containing amitriptyline HCl and perphenazine.
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