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Simultaneous estimation of Ritonavir and Lopinavir in pharmaceutical dosage form by RP-HPLC method

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Author(s): K. Anand Babu1*, B. Jayakar1, G.Vinoth kumar2.

Journal: Journal of Pharmacy Research
ISSN 0974-6943

Volume: 4;
Issue: 9;
Start page: 2985;
Date: 2011;
Original page

Keywords: Ritonavir | Lipinavir | HPLC | Method Development.

ABSTRACT
A simple, rapid, accurate and precise reverse phase high performance liquid chromatographic method has been developed for the simultaneous determinationof Ritonavir and Lopinavir in pharmaceutical dosage forms. Chromatography was carried out on a C18 column using a mixture of ammonium acetate andacetonitrile in the ratio of 55:45 v/v as the mobile phase at a flow rate of 1.5 ml/min. and eluents are monitored at 210 nm. The calibration curves were linearover the range of 12.5ppm to 75ppm for Ritonavir and for Lopinavir 50 to 300ppm. The retention times of Ritonavir and Lopinavir was found to be 10.69and 13.23 min., respectively. The intra and inter day variation was found to be less than 1% showing high precision of assay method. Due to its simplicity,rapidness and high precision, the proposed HPLC method may be used for simultaneous determination of these two drugs in pharmaceutical dosage forms.
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