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Simultaneous two-photon activation of presynaptic cells and calcium imaging in postsynaptic dendritic spines

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Author(s): Matsuzaki Masanori | Ellis-Davies Graham CR | Kanemoto Yuya | Kasai Haruo

Journal: Neural Systems & Circuits
ISSN 2042-1001

Volume: 1;
Issue: 1;
Start page: 2;
Date: 2011;
Original page

ABSTRACT
Abstract Background Dendritic spines of pyramidal neurons are distributed along the complicated structure of the dendritic branches and possess a variety of morphologies associated with synaptic strength. The location and structure of dendritic spines determine the extent of synaptic input integration in the postsynaptic neuron. However, how spine location or size relates to the position of innervating presynaptic cells is not yet known. This report describes a new method that represents a first step toward addressing this issue. Results The technique combines two-photon uncaging of glutamate over a broad area (~500 × 250 × 100 μm) with two-photon calcium imaging in a narrow region (~50 × 10 × 1 μm). The former was used for systematic activation of layer 2/3 pyramidal cells in the rat motor cortex, while the latter was used to detect the dendritic spines of layer 5 pyramidal cells that were innervated by some of the photoactivated cells. This technique allowed identification of various sizes of innervated spine located
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Tango Jona
Tangokurs Rapperswil-Jona