Academic Journals Database
Disseminating quality controlled scientific knowledge

Single-cell time-lapse analysis of depletion of the universally conserved essential protein YgjD

ADD TO MY LIST
 
Author(s): Bergmiller Tobias | Peña-Miller Rafael | Boehm Alexander | Ackermann Martin

Journal: BMC Microbiology
ISSN 1471-2180

Volume: 11;
Issue: 1;
Start page: 118;
Date: 2011;
Original page

ABSTRACT
Abstract Background The essential Escherichia coli gene ygjD belongs to a universally conserved group of genes whose function has been the focus of a number of recent studies. Here, we put ygjD under control of an inducible promoter, and used time-lapse microscopy and single cell analysis to investigate the phenotypic consequences of the depletion of YgjD protein from growing cells. Results We show that loss of YgjD leads to a marked decrease in cell size and termination of cell division. The transition towards smaller size occurs in a controlled manner: cell elongation and cell division remain coupled, but cell size at division decreases. We also find evidence that depletion of YgjD leads to the synthesis of the intracellular signaling molecule (p)ppGpp, inducing a cellular reaction resembling the stringent response. Concomitant deletion of the relA and spoT genes - leading to a strain that is uncapable of synthesizing (p)ppGpp - abrogates the decrease in cell size, but does not prevent termination of cell division upon YgjD depletion. Conclusions Depletion of YgjD protein from growing cells leads to a decrease in cell size that is contingent on (p)ppGpp, and to a termination of cell division. The combination of single-cell timelapse microscopy and statistical analysis can give detailed insights into the phenotypic consequences of the loss of essential genes, and can thus serve as a new tool to study the function of essential genes.
Why do you need a reservation system?      Affiliate Program