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Single RP-HPLC method for the determination of Hydrochlorothiazide, Amlodipine besylate and Valsartan in pharmaceutical products

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Author(s): Kullai Reddy Ulavapally1, J. Sriramulu1, Viswanath Reddy Pyreddy1 and Varaprasad Bobbarala2*.

Journal: Journal of Pharmacy Research
ISSN 0974-6943

Volume: 4;
Issue: 3;
Start page: 894;
Date: 2011;
Original page

Keywords: Amlodipine besylate | Valsartan | Hydrochlorothiazide and RP-HPLC method.

ABSTRACT
A simple and accurate RP-HPLC method has been developed for the simultaneous estimation of Hydrochlorothiazide, Amlodipine besylate and Valsartan by using C18 column 150 x 4.6 mm, 5μm with a simple gradient elution(0-4min, sol-A:80-55; 4-8min- sol-A:55-40; 8-11min- sol-A:40-30; 11-13min- sol-A:30-80 and 13-16min- sol-A:80-80). Mobile phase comprising of sol- A (pH 3.00±0.05 of 0.01M Potassium dihydrogen phosphate) and sol-B (Acetonitrile). Flow rate was 1.00 ml per min and the detection was monitored out by UV detector at 237nm. The retention times for Hydrochlorothiazide, Amlodipine besylate and Valsartan were found 4.5, 6.0 and 10.6 minutes. The proposed method has permitted the quantification of Hydrochlorothiazide, Amlodipine besylate and Valsartan over linearity in the range of 5-75 μg per ml and applicable for bulk and all type of pharmaceutical dosage forms.
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