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A Single Step Purification of Gastricsin-Like Proteinase from Atlantic Cod (Gadus morhua)

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Author(s): M. A. Amiza | R.K. Owusu Apenten

Journal: Journal of Biological Sciences
ISSN 1727-3048

Volume: 2;
Issue: 9;
Start page: 591;
Date: 2002;
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Keywords: Enzyme | purification | cod | cod | protease

ABSTRACT
A gastricsin-like proteinase was purified from gastric mucosa of Atlantic cod by a single step purification scheme on ion-exchange of Amberlite CG-50. The purification was very efficient as the recovery was 205% and the purification factor was 1796-fold. The enzyme preparation was homogeneous as observed by SDS-PAGE and isoelectric focusing. The enzyme has an estimated molecular weight of 34 kDa and the pI of 4.4.
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