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SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF FLUNIXIN MEGLUMINE AND MENBUTONE IN BULK AND DOSAGE FORMS

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Author(s): FOUAD M.M. | ABD EL-RAZEQ S.A. | BELAL F.F. | FOUAD F.A.

Journal: International Journal of Pharmaceuticals Analysis
ISSN 0975-3079

Volume: 4;
Issue: 1;
Start page: 30;
Date: 2013;
Original page

Keywords: flunixin meglumine | menbutone | charge-transfer | naphthoquinone-4-sulfonate | ion pair | KIO3/KI.

ABSTRACT
Four simple, accurate and reproducible spectrophotometric methods are presented for the determination of two veterinary drugs, flunixin meglumine (flunixin-M) and menbutone in pure form as well as in pharmaceutical dosage forms. The first one (Method A) depends on the formation of a charge-transfer complexes between flunixin-M as n-donor and 2,3-dicholro-5,6-dicyano-1,4-benzoquinone (DDQ), chloranilic acid (CA) and p-Chloranil (CR) as p-acceptors giving colored products quantified spectrophotometrically at 588, 521 and 345 nm, respectively. Beer's plot shows good correlations in the concentration range of 10-100, 50-300 and 10-80 µg mL-1, respectively. The second method (Method B) depends on the nucleophilic substitution reaction between the cited drug and 1,2-naphthoquinone-4-sulfonate (NQS) producing an orange-red colored product measured at 484 nm with a linearity range of 20-120 µg mL-1. The third method (Method C) is based on the formation of ion pair complexes between menbutone and basic fuchsin (BF), methylene blue (MB) and safranin O (SFNO) in buffered aqueous solution at pH 7.5 ±0.5 with the three dyes to give highly colored complex species extractable with chloroform, measured at 557, 650 and 516 nm, respectively. Beer's law is obeyed in the concentration range of 8-40 µg mL-1 with BF and SFNO or 1-8 µg mL-1 with MB. The fourth method (Method D) is based on the reaction of carboxylic acid group of menbutone with a mixture of potassium iodate and potassium iodide in aqueous medium at room temperature producing yellow free I2 having a maximum at 350 nm with a linearity range of 3-20 µg mL-1. The suggested methods are used to determine the cited drugs in pharmaceutical formulation; flunidyne or menbutone injections with mean recoveries ranging from 98.98-100.99%. The validity of the methods is further assessed by applying the standard addition technique. The obtained results are statistically analyzed and found to be in accordance with those given by manufacturer methods.
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