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Spontaneous and induced frequency of chromosomal aberration in bone marrow of NMRI and Balb-C mice of both sexes

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Author(s): Daniel Francisco Arencibia Arrebola | Luis Alfredo Rosario Fernández | Yanet Rodriguez | Yulieé López Feria | Daiyana Díaz Rivero

Journal: RETEL : Revista de Toxicología en Línea
ISSN 1668-091X

Volume: 23;
Start page: 8;
Date: 2009;
Original page

Keywords: Spontaneous | induced | chromosomal aberration | NMRI mice | Balb-C mice | cyclophosphamide | bone marrow

ABSTRACT
The in vivo citogenetic assay is a short term mutagenicity assay, of great sensibility, useful to detect structural chromosomal aberrations fundamentally under conditions that they involve the answer the in vivo response, they can vary according to the species and the tissue, they are generally evaluated during the first serial mitosis to the treatment. In this method, are used cells of bone marrow of mammals to be more vascularisated and to contain a cells population of short cycle that they can be isolated and to easiness. In this article is reported the basal frequency of appearance of chromosomal aberrations in of the bone marrow cells of NMRI and Balb-C mice of both sexes, as well as the induced frequency of events, after the cyclophosphamide (CF) administration for intraperitoneal route, reference mutagen used as positive control. You could conclude that the NMRI and Balb-C mice, constitute a good model to use in the genotoxicologic studies, given the spontaneous drop frequency of structural type of aberrations, the mitotic index and the number of polyploidy cells, as well as we stood out the sensibility to the cyclophosphamide action of the administered in dose of 50 mg/kg for intraperitoneal route at the 48 and 24 hours before the sacrifice.
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