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Sprague Dawley response to the cyclophosphamide and bleomycin in the alkaline comet assay of peripheral blood leukocytes

Author(s): Daniel Francisco Arencibia Arrebola | Alexis Vidal Novoa | Luis Alfredo Rosario Fernández | Yolanda Emilia Suárez Fernández

Journal: RETEL : Revista de Toxicología en Línea
ISSN 1668-091X

Volume: 30;
Start page: 21;
Date: 2010;
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Keywords: Alkaline comet assay | peripheral blood leukocytes | Sprague Dawley | cyclophosphamide | bleomycin

In this article we decide to evaluate genotoxic effect of the cyclophosphamide and bleomycin in the individual cells by means of alkaline comet assay using the Sprague Dawley rats in both sexes as experimental biomodel. Which were formed 5 experimental groups per sex, the first administered with NaCl 0,9 % by intraperitoneal (i.p) route, the second and third groups were administered with cyclophosphamide by i.p route, with designs of different treatments at doses of 50 mg/kg. The fourth and fifth groups were administered with bleomycin by i.p route, equally in two designs of different treatments at doses of 40 mg/kg. At the end of the experience bigger induction of damage was obtained with the use of the cyclophosphamide and bleomycin, both in the design of 48 and 24 hours administration before the sacrifice. This constitutes under our experimental conditions the two better experimental designs to induce the strand breaks (SB) or alkali-labile sites formation on DNA, increasing considerably the frequency spontaneous present in this species of rat, being useful in studies of drugs evaluation that they have not been explored in to the in vivo antigenotoxicity and genotoxicity environment.
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