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Starvation of Jurkat T cells causes metabolic switch from glycolysis to lipolysis as revealed by comprehensive GC-qMS

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Author(s): Janina M. Tomm | Wolfgang Otto | Ulrich Servos | Bernd Bertram | Martin von Bergen | Sven Baumann

Journal: Journal of Integrated OMICS
ISSN 2182-0287

Volume: 3;
Issue: 1;
Start page: 70;
Date: 2013;
Original page

Keywords: Comprehensive GC-qMS | Metabolite profiling | Starvation | Jurkat T cells

ABSTRACT
T cells play a central role in the cellular part of the immune system and their metabolomic activity is strictly bound to the status of activation. Little is known about the metabolomic resilience of T helper cells in the resting status. Therefore we analyzed the metabolomic profile of non-activated Jurkat T cells under normal and starvation conditions by a two-dimensional GC-MS approach. We detected 52 organic and amino acids in the biological replicates covering the majority of central metabolic pathways. Under starvation conditions 21 analytes representing major metabolic pathways showed a significant down-regulation. For palmitoleic acid a significant up-regulation was detected. The annotation of differentially abundant metabolites to the pathways of TCA-cycle, amino acid metabolism and fatty acid biosynthesis indicating a metabolic switch from glycolytic to lipolytic energy generation upon starvation.
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