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Strategic marker selection to detect quantitative trait loci in chicken

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Author(s): Ruy Deborah Clea | Nones Kátia | Baron Erica Elias | Ledur Mônica Corrêa | Melo Cláudio Manoel Rodrigues de | Ambo Marcel | Campos Raquel de Lello Rocha | Coutinho Luiz Lehmann

Journal: Scientia Agricola
ISSN 0103-9016

Volume: 62;
Issue: 2;
Start page: 111;
Date: 2005;
Original page

Keywords: QTL | broiler | selective genotyping | body weight

ABSTRACT
Selective genotyping for a certain trait in individuals with extreme phenotypes contributes sufficient information to determine linkage between molecular markers and quantitative trait loci (QTL). In this experiment an F2 population, developed by crossing males from a broiler line with females from a layer line, was employed to detect QTL on chromosomes 3 and 5. Twenty-eight performance and carcass traits were measured in F2 offspring, and phenotypic correlations between traits were calculated. Body weight at 42 days (BW42) presented the greatest positive correlations with most other traits, with correlation between body weights at 35 and 41 days, weight gain between birth and 35, 41 and 42 days, as well as weights of carcass and some body parts superior to 0.8. One hundred-and-seventy F2 offspring, representing the top (4.5%) and the bottom (4.5%) of a normal distribution curve of BW42, were selected with equal proportions of males and females, and within dam family. Samples were genotyped for 19 informative markers on chromosome 3, and 11 markers on chromosome 5. Marker allelic frequencies of phenotypic groups with high and low BW42 were compared with a chi-square test. Four regions on chromosome 3 and three regions on chromosome 5 had markers that were suggestively associated with BW42 (P < 0.10), confirming and expanding previous studies.
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