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Structural Effects of Vitamin E on Proximal Tubule and Interstitium in a Rat Model of Cyclosporin A Nephrotoxicity

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Author(s): Yesim Hulya Uz | Muberra Uygun

Journal: Pakistan Journal of Biological Sciences
ISSN 1028-8880

Volume: 8;
Issue: 12;
Start page: 1712;
Date: 2005;
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Keywords: vitamin E | Cylosporin A | kidney | proximal tubule | interstitium

ABSTRACT
The aim of this study was to investigate whether the antioxidant vitamin E provided morphological protection in the nephrotoxicity caused by Cyclosporin A (CsA). Twenty-five Sprague-Dawley male rats were divided into five groups, each consisting of five rats. One of them was the control group and four of them were the experiment group. CsA (5 mg kg-1 day-1) to the Group II, III and V, CsA plus vitamin E (5 mg kg-1 day-1) to the Group IV were administered intraperitoneally (i.p.) for 8 weeks. The kidney specimens of the Group I, II and IV were taken at the end of the 8th week. The kidney specimens of the Group III were taken at the end of 14th week after they had been kept for reversibility for 6 weeks. As for t he kidney specimens of the Group V, they were administered vitamin E ( 5 mg kg-1 day-1) for 6 weeks after the administration of CsA (5 mg kg-1 day-1) for 8 weeks and then were taken at the end of the 14th week. All of these kidney specimens were processed for light and electron microscopical examination. In the Group II, infiltration foci and increase of interstitial connective tissue were observed at the surrounding of vessels, especially in the corticomedullary region. The most obvious changes were encountered in the proximal tubules. These changes were seen as degeneration and regeneration. While the degeneration was seen as the thickening of basement membrane, loss of brush border, vacuolization, dilation of the smooth endoplasmic reticulum, increase in lysosomes in number and size, the proliferation of some of the scattered epithelial cells of the tubules formed the regeneration areas by causing the appearance of new tubules. No obvious regression was seen in the Group III and more or less the same changes as in the Group II were observed. As vitamin E inhibited the oxidative damage of CsA, the least damage occurred in the Group IV. Because of the release of CsA accumulated in tissues later giving it into the organism, more damage was observed in the Group V compared to Group IV. Therefore, using CsA and vitamin E simultaneously may keep the nephrotoxicity caused by CsA at a minimal level.
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