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Studies on Isolation, Purification and Molecular Identification of Pectinase Producing Bacteria

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Author(s): Dr. B. Lalitha kumari | R. Lalitha | Dr. P. Sudhakar

Journal: International Journal of Advanced Research
ISSN 2320-5407

Volume: 1;
Issue: 5;
Start page: 204;
Date: 2013;
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Keywords: Pectinolytic bacteria | biochemical tests | sequencing | genomic DNA | amplification | SEM | Pectinase.

ABSTRACT
Pectin is a structural polysaccharide present in primary cell wall and middle lamella of fruits and vegetables. Pectin or pectic substances are heterogeneous group of high molecular weight, complex, acidic structural polysaccharides with a backbone of galacturonic acid residues linked by α-(1-4) linkages. Pectins show widespread commercial uses. Pectinases are a group of enzymes that breakdown pectins. Because of the potential and wide applications of pectinases, there is a need to highlight recent developments on several aspects related to their production. However, aspects regarding the most common microorganisms and processes for hydrolytic depolymerising pectinase (PGase) production have not been considered until now. The present study was undertaken to isolate bacteria from soil samples that produce pectinase enzyme using a selective medium after enrichment and to identify the bacterial isolates based on biochemical and molecular characterization by sequencing the 16S rRNA coding gene and based on the 16s rRNA sequence, the culture was identified as Bacillus subtilis.
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