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Studies on Phospholipase A from Melia azadirachta (Neem) Seeds Extract

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Author(s): M. Umar Dahot | A.B.Ghanghro | M. Yousuf Khan

Journal: Journal of Biological Sciences
ISSN 1727-3048

Volume: 1;
Issue: 8;
Start page: 788;
Date: 2001;
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Keywords: Enzyme | phospholipase A | melia azadirachta | seeds

ABSTRACT
The crude extract from dried Melia azadirachta seeds was prepared and it was noted that the seed contains sufficient amount of phospholipase A activity. Optimal activity of Melia azadirachta seeds was found at pH 6.0 and at temperature 35�C. The stability of enzyme was noted over the pH range 5-8. More than 30% activity of phospholipase A remained active up to 60 minutes at 60�C. The enzyme activity was markedly increased in the presence of calcium, manganese and zinc but it was decreased by the addition of EDTA.
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