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Studies regarding chaotropic effects of sodium perclorate on Zymomonas mobilis bacterial membrane

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Author(s): Andrei Tanase | Cristina Sturzoiu | Iulia Florescu | Gheorghe Stoian

Journal: Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara
ISSN 1582-3571

Volume: 11;
Issue: 4;
Date: 2010;
Original page

ABSTRACT
Chaotropic agents decrease the strength of hydrophobic interactions between nonpolar compounds. The aim of this study is to present the chaotropic effects of sodium perchlorate on bacterial membrane. The membrane permeabilization by chaotropic treatment was highlighted using PAGE electrophoresis and fluorescent microscopy with hypericin from Hypericum perforatum as an effect marker. Electrophoresis for membrane enzyme activity of ALP, ATP-ase, G6PDH and GFOR revealed perchlorate effect. The two ADH isoforms deserve further attention. This technique can be useful in membrane enzymes extraction in biotechnological purpose. On the other hand, since Z. mobilis is very difficult for genetic manipulation, the use of NaClO4 can be a solution for membrane permeabilization in perspective of genetic engineering.
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