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The study of genetic diversity within Carassius genera, based on sequencing some mitochondrial markers

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Author(s): Mihaela-Liliana IONESCU | Gogu I. GHIORGHITA

Journal: Analele Universitatii din Oradea, Fascicula Biologie
ISSN 1224-5119

Volume: TOM XX;
Issue: 1;
Start page: 29;
Date: 2013;
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Keywords: D-loop | Cyt b | COX I (CO I) | Carassius

ABSTRACT
In this study we investigated the genetic diversity within Carassius genera, studying individuals from isolated aquatic populations in Romania, by analysing the sequences of three mitochondrial DNA genes: cytochrome b (Cyt b), mitochondrial control region (D-loop) and cytochrome c oxidase I (COX I). The nucleotide sequence variation of the three genes were used to study the mtDNA divergence for Carassius genera individuals and to examine the phylogenetic relationships within analyzed populations.Based on the alignment of cytochrome b gene sequences from individuals belonging to Carassius genera from analyzed populations, 21 haplotypes have been identified: two of them were found in four of the six analyzed populations and one in two of studied populations. Regarding the D-loop sequences there were identified 20 haplotypes: four of them were found in two or more populations. Following COX I sequence alignment, from individuals of the Carassius genera, in the six populations were identified 22 haplotypes, but only one was found in four of the analyzed populations. Phylogeographic aspects of the D-loop showed that there are common haplotypes between Buzău (Buzău River, Buzău County, Romania), Sofroneşti (Sofroneşti Lake, Vaslui County, Romania) Delta (Fortuna Lake, Danube Delta, Romania) and Băile Felix (Bihor County, Romania) populations, and for COX I between Buzău (Buzău River, Buzău County, Romania), Tăuteşti (Tăuteşti Lake, Iaşi County, Romania), Delta (Fortuna Lake, Danube Delta, Romania) and Băile Felix (Bihor County, Romania) populations. From the analysis of all sequences, it was found that the rate of occurrence of transitions is greater than the occurrence of transversions.
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