Academic Journals Database
Disseminating quality controlled scientific knowledge

Study on clinical and laboratory diagnostic of Lyme disease in dogs after experimental infection

Author(s): Savić Sara | Vidić Branka | Grgić Ž. | Milanov Dubravka | Stojanović Dragica | Šeguljev Zorica

Journal: Acta Veterinaria
ISSN 0567-8315

Volume: 62;
Issue: 2-3;
Start page: 343;
Date: 2012;
VIEW PDF   PDF DOWNLOAD PDF   Download PDF Original page

Keywords: diagnostic methods | dogs | experimental infection | Lyme borreliosis

Experimental infection was done on 13 dogs, with B. burgdorferi s.l., in the epitzootiological area where Lyme disease in dogs and humans is present. Prior to the experimental infection, dogs in the experiment had no contact with B. burgdorferi, and they were kept in isolation. Serological methods used in the study were complement fixation and ELISA test. Biochemical blood analysis was done, also. The experimental infection of dogs was done with a referent ATCC B. burgdorferi s.l. culture, and with the isolates of B. burgdorferi s.l. previousely gained from Ixodes ricinus ticks collected on selected locations of the observed region in the northern part of Serbia (Vojvodina province). After the experimental infection, clinical symptoms were not seen in dogs and positive serological results were found in 70% of experimentally infected dogs. Immunodiagnostic criteria for the diagnosis of Lyme disease in dogs are established. In dogs without clinical symptoms for Lyme disease, when clarifying the laboratory results, one must have in mind the epizootiological situation of the region and also the possibility of former contact of the dog with B. burgdorferi s.l. For epizootiological surveys, CF can be used as an approximate screening method, with obligatory conformation with ELISA in the case of positive findings.
Affiliate Program      Why do you need a reservation system?