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A STUDY ON SEASONAL VARIATION IN CYTOTOXIC AND ANTIOXIDANT ACTIVITIES OF TERMINALIA BELLERICA

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Author(s): Sunita Bhatnagar | Sunita Sahoo | Dipti Ranjan Behera | A.K. Mohapatra

Journal: International Journal of Biomedical and Advance Research
ISSN 2229-3809

Volume: 2;
Issue: 11;
Start page: 427;
Date: 2011;
Original page

Keywords: Terminalia bellerica | extracts | DPPH | BEA | cytotoxicity and antioxidant

ABSTRACT
Study was conducted to ascertain medicinal potential of leaf and bark and the role of time period in collection of medicinal plant Terminalia bellerica on its cytotoxic and antioxidant potential. Terminalia bellerica, leaf and bark were collected in the months of February, May, August and October. Solvent extracts namely hexane, dichloromethyl, chloroform, acetone and methanol  were tested for cytotoxicity against brine shrimps at four doses 25, 50, 100 and 200µl/ml.  All the extracts barring few showed dose dependent activity and   marked differences were observed in different seasons. Thin layered chromatography based 2, 2 diphenyl-1-picrylhydrazyl (DPPH) assay for assessing the antioxidant potential was conducted using three different solvents, Maximum number of antioxidant bands were obtained in non polar basic solvent that is Benzene: ethanol: ammonium hydroxide(90:10:1)(BEA).. Quantitative radical scavenging assay was also conducted and methanol extract of leaf was found to show significant result to the tune of 100 percent at higher dose of 1250microgram/ml. The best season for the collection of medicinal plant was found to be from May to July on the basis of cytotoxic as well as antioxidant activities
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