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Study on separating, identification and fermentation characteristics in probiotics with inhibitory activity on angiotensin converting enzyme

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Author(s): YU Long-Hao | CHEN Hong-Sheng | HUO Guang | XUE Bing

Journal: Shipin Anquan Zhiliang Jiance Xuebao
ISSN 2095-0381

Volume: 4;
Issue: 1;
Start page: 289;
Date: 2013;
Original page

Keywords: anti-hypertension | angiotensin converting enzyme inhibition rate | strain screening | fermentation characteristics | identification

ABSTRACT
Objective To screen out microbes which have inhibition effect on angiotensin converting enzyme (ACE) for providing the strains which may decrease the blood pressure. Methods After separated from seven kinds of fermented food, the objective strains whose ACE inhibition rate was more than 80% were screened out by the experiments of ACE inhibition in vitro, and the salt tolerance and nitrite tolerance, as well as molecular biology and biological characteristics were determined. Results Ninety-eight kinds of strains from 7 kinds of fermented food were separated and screened. Among them, the ACE inhibition rate of F2 and D2 were 83.13% and 98%, respectively, and they could tolerate 6% NaCl and 100 mg/kg nitrite. Conlusion F2 is Bacillius licheniformis and it has antibacterial effect on Staphylococcus aureus, and D2 is Aneurinibacillus miqulanus and it has lipase activity, and they all have protease activity.
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