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Study of Photoinduced Interaction between Calf Thymus-DNA and Bovine Serum Albumin Protein with H<sub>2</sub>Ti<sub>3</sub>O<sub>7</sub> Nanotubes

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Author(s): Rajesh Chakraborty | Sriparna Chatterjee | Sandipan Sarkar | Pabitra Chattopadhyay

Journal: Journal of Biomaterials and Nanobiotechnology
ISSN 2158-7027

Volume: 03;
Issue: 04;
Start page: 462;
Date: 2012;
Original page

Keywords: Photoinduced Interaction | Nanotubes | Hydrothermal Process

ABSTRACT
Hydrogen titanate nanotubes were synthesized by hydrothermal process using 10 M NaOH and TiO2 anatase powder. The material synthesized was characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to ensure the structural and morphological characteristics. The interaction of calf thymus DNA (CT-DNA) and bovine serum albumin protein with suspended aqueous solution of titanate nanotubes was investigated using UV absorption spectroscopy and the apparent association constant was found to be, Kb= 1.68 × 104 M-1 and Kap=5.41 × 103 M-1 for DNA and BSA respectively. Addition of the titanate nano material resulted quenching of fluorescence spectra of ethidium bromide-DNA in tris HCl buffer solution and that of aqueous protein solution. The apparent binding constant (Ksv= 5.46 × 104M-1 for DNA binding and Ksv = 6.063 × 103M-1 for protein binding) was deduced from relevant fluorescence quenching data using Stern-Volmer equation.
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