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Supercritical Carbon Dioxide Extraction of the Oak Silkworm (Antheraea pernyi) Pupal Oil: Process Optimization and Composition Determination

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Author(s): Wen-Juan Pan | Ai-Mei Liao | Jian-Guo Zhang | Zeng Dong | Zhao-Jun Wei

Journal: International Journal of Molecular Sciences
ISSN 1422-0067

Volume: 13;
Issue: 2;
Start page: 2354;
Date: 2012;
Original page

Keywords: supercritical carbon dioxide | oak silkworm pupal oil | response surface methodology

ABSTRACT
Supercritical carbon dioxide (SC-CO2) extraction of oil from oak silkworm pupae was performed in the present research. Response surface methodology (RSM) was applied to optimize the parameters of SC-CO2 extraction, including extraction pressure, temperature, time and CO2 flow rate on the yield of oak silkworm pupal oil (OSPO). The optimal extraction condition for oil yield within the experimental range of the variables researched was at 28.03 MPa, 1.83 h, 35.31 °C and 20.26 L/h as flow rate of CO2. Under this condition, the oil yield was predicted to be 26.18%. The oak silkworm pupal oil contains eight fatty acids, and is rich in unsaturated fatty acids and α-linolenic acid (ALA), accounting for 77.29% and 34.27% in the total oil respectively.
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