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Supercritical CO2 extract and essential oil of bay (Laurus nobilis L.) – chemical composition and antibacterial activity

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Author(s): JASNA IVANOVIĆ | DUŠAN MIŠIĆ | MIHAILO RISTIĆ | OLIVERA PEŠIĆ | IRENA ŽIŽOVIĆ

Journal: Journal of the Serbian Chemical Society
ISSN 0352-5139

Volume: 75;
Issue: 3;
Start page: 395;
Date: 2010;
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Keywords: Laurus nobilis | bay | supercritical extraction | essential oil | antibacterial activity | gas chromatography.

ABSTRACT
The present study deals with the supercritical carbon dioxide (SC-CO2) extraction and hydrodistillation (HD) of dried bay leaves (Laurus nobilis L.). The chemical composition and antibacterial activity of the SC-CO2 extract and essential oil (EO) from dried leaves of bay were compared to each other and literature data. Qualitative and quantitative analyses of the SC-CO2 extract and EO were performed using GC–FID and GC–MS analytical methods. A significant difference in the chemical composition of the SC-CO2 extract and EO was observed. The EO comprised high contents of monoterpenes and their oxygenated derivates (98.4 %), principally 1,8-cineole (33.4 %), linalool (16.0 %) and α-terpinyl acetate (13.8 %), sabinene (6.91 %) and methyl eugenol (5.32 %). The SC-CO2 extract comprised twice less monoterpenes and their oxygenated derivates (43.89 %), together with sesquiterpenes (12.43 %), diterpenes (1.33 %) and esters (31.13 %). The major components were methyl linoleate (16.18 %), α-terpinyl acetate (12.88 %), linalool (9.00 %), methyl eugenol (8.67 %), methyl arachidonate (6.28 %) and eugenol (6.14 %). An investigation of the antibacterial activity of bay SC-CO2 extract and EO was completed on different Staphylococcus strains using the broth macrodilution method. Staphylococcus intermedius strains were the most susceptible to both the SC-CO2 extract and EO (MIC = 640 µg/ml).
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