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Susceptibility Testing of Clinical Mould Isolates in Malaysia with the E-test and M38-A Methods

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Author(s): Jacinta Santhanam | Ang Kok Teong | Fairuz Amran

Journal: Journal of Medical Sciences
ISSN 1682-4474

Volume: 8;
Issue: 1;
Start page: 94;
Date: 2008;
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Keywords: Susceptibility | fungi | E-test | M38-A | agreement | Malaysia

ABSTRACT
Susceptibility testing of filamentous fungal isolates was carried out with the aim of obtaining susceptibility data for Malaysian clinical isolates and to determine the agreement between two methods of susceptibility testing. Fifteen fungal isolates comprising of Aspergillus sp., Fusarium sp. and Cladosporium sp. were evaluated for susceptibility to amphotericin B with E-test and M38-A broth microdilution methods. Susceptibility to itraconazole and voriconazole was also determined with E-test method. With the M38-A method, nine Aspergillus sp. and two Cladosporium sp. isolates were found to be sensitive (MIC 2 μg mL-1), while three Fusarium sp. isolates appeared resistant (MIC ≥ 8 μg mL-1) to Amphotericin B. The E-test showed MIC values which were much lower (0.002-0.5 μg mL-1) for six Aspergillus sp. isolates and the two Cladosporium sp. isolates, while for Fusarium sp., MIC was ≥ 2 μg mL-1. Almost all isolates, other than Fusarium sp., were uniformly more susceptible to voriconazole (MIC: 0.008-0.5 μg mL-1) than itraconazole (MIC: 0.006-6 μg mL-1). In conclusion, Fusarium sp. showed least susceptibility to all antifungals tested. E-test and M38-A methods generated low agreement in determining MIC of amphotericin B and the agreement between the two test results was highest when results were read at 72 h incubation.
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