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Targeting and genotyping RGAs in a mappingpopulation of the AA genome of wild Arachis

Author(s): Soraya Cristina de Macedo Leal-Bertioli | Patrícia Messenberg Guimarães | David John Bertioli

Journal: Crop Breeding and Applied Biotechnology
ISSN 1518-7853

Volume: 7;
Issue: 2;
Start page: 179;
Date: 2007;
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Keywords: Peanut | molecular markers | resistance gene analogs | marker-assisted selection | Arachis.

Peanut is an important legume crop. Although it has high levels of morphological diversity, it lacks geneticvariability and sources of disease resistance. The transference of resistance genes from wild species is difficult due to thedifferent ploidy level of the wild and cultivated species. Recently, amphidiploids have been produced that can be used as‘bridges’ to introgress wild genes. Molecular markers are useful to pyramidize desirable genes and track them throughgenerations of backcrossings. Molecular markers based on Resistance Gene Analogs have improved chances to be present inor linked to resistance gene loci. This study describes the development and genotyping of molecular markers based onresistance gene motifs. Specific primers were designed based on unique sequences of an Arachis RGA dataset. The identity ofthe amplified polymorphic bands was confirmed by sequencing. These markers were genotyped on a F2 population thatsegregates for resistance to biotic stress types.
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