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Therapeutic effects of all-trans retinoic acid on experimental autoimmune encephalomyelitis and its role in T-helper lymphocyte responses

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Author(s): Abtahi Froushani SM | Delirezh N | Hobbenaghi R | Mosayebi Gh

Journal: Tehran University Medical Journal
ISSN 1683-1764

Volume: 69;
Issue: 11;
Start page: 710;
Date: 2012;
Original page

Keywords: All-trans-retinoic acid | autoimmune response | experimental autoimmune encephalomyelitis | lymphocyte | multiple sclerosis

ABSTRACT
"nBackground: Recent studies have demonstrated an essential role for IL-17 in the pathogenesis of experimental autoimmune encephalomyelitis (EAE). Furthermore, it has been shown that FoxP3+Treg cells play an important role in the suppression of autoinflammatory reactions. Although, previous studies have determined the immunomodulatory potentials of all-trans-retinoic acid (ATRA), but these immunomodulations have been mostly justified by alteration in Th1/Th2 cytokines. The present study was carried out to investigate the therapeutic effects of ATRA on EAE and its effects on T-helper cells responses."n "nMethods: EAE was induced by MOG35-55 peptide and complete Freund's adjuvant in female C57BL/6 mice. The mice were allocated to two therapeutic groups (n=7 per group). Treatment with ATRA (500 μg/mouse; every other day) was initiated in treatment group on day 12 when they developed a disability score. EAE controls received vehicle alone with the same schedule. Signs of disease were recorded daily until day 33 when the mice were sacrificed. Splenocytes were tested for proliferation by MTT test, cytokine production by ELISA and FoxP3+Treg cell frequency by flowcytometry."n "nResults: ATRA significantly reduced the clinical signs of established EAE. Aside from decreasing lymphocytic proliferation (P

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