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Tracheobronchial Tree Variations: Study Methodology

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Author(s): Viscuso, Matías N. | Arcamone, María L. | Corrado, Mariana | Piscopo, Agustín

Journal: Revista Argentina de Anatomia Online
ISSN 1853-256X

Volume: 2;
Issue: 1;
Start page: 15;
Date: 2011;
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Keywords: Tracheobronchial Tree | Study Methodology | Injection | Silicone | Didactic material | Variations

ABSTRACT
The study of the tracheobronchial tree and bronchopulmonary segmentation presents challenges due to the intraparenchymatous nature of the majority of its bronchi. Scan studies, allow through its tridimensional reconstructions the analysis of the intrapulmonary structures. However, they incur in high costs which make them difficult to realize.This work introduces an alternative method to the study of the bronchial tree, with five stages applicable to humans and rest of the mammals, characterized for being inexpensive, simple, brief and effective. As an example, the domestic rabbit (oryctolagus cunniculus) has been used to illustrate the method. The study focuses on the injection of the bronchial tree using silicone to obtain molded pieces that can be used for further analysis or as learning material.1) Stage of dissection: 21 adult rabbits, no sex distinction, cardiopulmonary blocks were removed. 2) Stage of silicone injection: the tracheobronchial tree lumen was filled with semi-solid silicone. 3) Stage of maceration: the preparations were placed in a hydrogen peroxide solution in order to decompose the biological tissues. 4) Data gathering phase: in order to follow a structured process for observation the molded pieces were numbered and analyzed according to an "observer's guide" that establishes the proper order for the study of variations. 5) Data processing and conclusion drawing phase: a digital spreadsheet was used to obtain the results that were then compared to the established bibliography.During this study there are registered: 4 (19%) development variations, 3 (14,3%) supernumerary bronchi, 2 (9,5%) hypoplasias, 1 (4,8%) agenesia.This method provides the means to properly and efficiently deliver results that are consistent with the related bibliography. We believe it's a useful method for the study of human, veterinarian and compared anatomy. Additionally the products created by the method can be used as tridimensional learning resources.
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