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Transcriptomic responses in Japanese medaka (Oryzias latipes) exposed to 17β-estradiol

Author(s): Mizukami-Murata Satomi | Kishi-Kadota Katsuyuki

Journal: Journal of Integrated OMICS
ISSN 2182-0287

Volume: 1;
Issue: 1;
Start page: 180;
Date: 2011;
Original page

Keywords: Affinity chromatography | 17β-estradiol | medaka | DNA microarray | Endocrine disruption | Feminization

The effects of 17β-estradiol (E2) were evaluated using the medaka DNA microarray representing 36,398 genes. We first evaluated chronic ef-fects on medaka exposed to E2 at different concentrations for 60 days posthatch. At ≥ 30 ng/L of E2 severe reproductive impairments such as sex reversal were observed. Larval medaka, Oryzias latipes, (within 24 hrs posthatch) were then exposed to E2 at various concentrations (3, 30, 100 ng/L) for up to 7 days. Microarray analyses of the E2-exposed larvae revealed that exposure to E2 up-regulated and down-regulated 339 and 105 genes, respectively. The up-regulated genes included ones involved in the p53 signaling pathway, apoptosis, and growth and develop-ment, in addition to well-known biomarkers such as vitellogenin and choriogenins. Down-regulated genes included heat shock proteins and estrogen receptors. Most of the up-regulated genes encoding the p53 signaling pathway, apoptosis, and growth and development exhibited a dose-dependent increase in gene expression, whereas the down-regulated genes in the heat shock protein category showed a dose-dependent decrease in gene expression. Time course experiments suggested that the E2 treatment attenuated the time-dependent changes in gene expres-sions of these genes. Among the genes related to oocyte maturation, estrogen-regulated genes such as choriogenins and vitellogenins were dramatically induced in response to E2 exposure, whereas other steroid-regulated genes such as zona pellucida-domain proteins did not change in gene expression by the E2 treatment. Results suggest that transcriptomic studies on larval medaka help elucidate the effects caused by endo-crine disruptors on various biological pathways in vertebrate development.
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