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Transforming growth factor β1 mRNA expression in mouse gonads and the effect of serine/threonine or glycine on its expression

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Author(s): Hüseyin Baki Çiftci

Journal: Research Opinions in Animal & Veterinary Sciences
ISSN 2221-1896

Volume: 1;
Issue: 7;
Start page: 441;
Date: 2011;
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Keywords: Mouse | mRNA | Ovary | Testis | TGF-β1

ABSTRACT
The objective of this study was to show transforming growth factor-β1 (TGF-β1) mRNA expression in mouse gonads and to show the changes in TGF-β1 mRNA expression after the injection of serine/thraonine mixture or glycine. Male and female mouse (19-21 days old, CD-1 strain, n=60) were divided into six groups and intraperitonealy injected for 5 days with saline (control), a mixture of serine/threonine or glycine (tests). After five days, the mice were killed and gonads were removed under RNAase free conditions. Total RNA was extracted and transferred on to a nylon membrane and hybridized with 32P-labelled cDNA probes. In both testis and ovary, a weakly expressed 5.2 kb spice of mRNA was detected and its expression was tended to increase by the injection of serine/threonine in ovaries and testes. The reason for increased TGF-β1 mRNA expression might be caused by increased intracellular concentration of glucose due to the serine/threonine or glycine injection.
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