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Transient expression in tobacco Bright Yellow 2 cells and pollen grains: A fast, efficient and reliable system for functional promoter analysis of plant genes

Author(s): Bratić Ana M. | Majić Dragana B. | Samardžić Jelena T. | Kragl M.W. | Maksimović Vesna R.

Journal: Archives of Biological Sciences
ISSN 0354-4664

Volume: 62;
Issue: 1;
Start page: 57;
Date: 2010;
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Keywords: Promoter | transient expression | BY2 cell | pollen grain

Gene expression is mediated by DNA sequences directly upstream from the coding sequences, recruited transcription factors and RNA polymerase in a spatially-defined manner. Understanding promoter strength and regulation would enhance our understanding of gene expression. The goal of this study was to develop a fast, efficient and reliable method for testing basal promoter activity and identifying core sequences within its pollen specific elements. In this paper we examined the functionality of buckwheat metallothionein promoter by a histochemical GUS assay in two transient expression systems: BY2 cells and pollen grains. Strong promoter activity was observed in both systems.
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