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Two-Phased Alteration and Clinical Relevance of MTA1 and MTA1s Gene Expression: A Quantitative PCR Analysis in Breast Cancer Patients

Author(s): Kung-Chia Young | Ching-Ting Huang | Shoei-Loong Lin | Wei-Chiang Hsiao

Journal: Journal of Cancer Molecules
ISSN 1816-0735

Volume: 2;
Issue: 2;
Start page: 79;
Date: 2006;
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Keywords: MTA1 | MTA1s | lymph node metastasis | estrogen receptor | real-time PCR | breast cancer

Aim: Metastasis-associated protein 1 (MTA1) and the short-form variant of MTA1 (MTA1s) have been demonstrated to modulate estrogen receptor (ER) alpha-mediated gene transactivation and signal transduction. Overexpression of MTA1 is associated with tumor invasion and migration in several types of human cancers originated from gastrointestinal system, liver and lung. In the present study, we tested the hypothesis that the levels of MTA1 and MTA1s expression might affect mammary carcinogenesis and clinical breast cancer characters from female patients in Taiwan. Methods: A quantity of MTA1 and MTA1s at RNA level was determined in surgically resected specimens of 47 breast cancer tissues and paired adjacent normal tissues by real-time PCR technology. We compared the clinical parameters and investigated for association with MTA1 and MTA1s expression. Results: Breast tumor tissues displayed significantly declining levels of both MTA1 (P < 0.001) and MTA1s (P < 0.001) compared to the normal matched samples, with a mean down-regulation of ~ 94%. The ratio of MTA1s/MTA1 remained constant in the paired tissues. The MTA1 level was significantly higher in the group with tumor size > 5 cm compared to that with tumor size < or = 5 cm (P = 0.050) and it was also significantly higher in the group with number of metastatic lymph nodes > or = 4 than that without lymph node metastasis (P = 0.032). In addition, MTA1s was significantly correlated with ER alpha expression (P < 0.001), and the amount of MTA1s in the tumors with nuclear expression of ER alpha was higher than that in the tumors without ER alpha expression (P = 0.042). Conclusion: Our data suggest that MTA1 and MTA1s regulate mammary carcinogenesis and breast cancer progression in Taiwanese women.
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