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Upregulation of glucose uptake in L8 myotubes by the extract from Lagerstroemia speciosa: a possible mechanism of action

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Author(s): Juntipa Purintrapiban

Journal: Maejo International Journal of Science and Technology
ISSN 1905-7873

Volume: 3;
Issue: 03;
Start page: 472;
Date: 2009;
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Keywords: Lagerstroemia speciosa | glucose uptake | GLUT1 | polyphenols | L8 myotubes

ABSTRACT
The leaf of Lagerstroemia speciosa L. is used as an anti-diabetic herbal remedy in many countries. In an attempt to discover mechanisms of action of the L. speciosa extract that stimulate glucose uptake, a cell-based radioactive assay of glucose uptake was performed using L8 muscle cells. In this study, the methanol fraction of L. speciosa leaves (LSE) contained a high level of phenolic compounds and showed strong capability to stimulate glucose uptake in a dose-dependent manner. The LSE stimulation was slightly inhibited (8.8%) by SB203580. The inhibitory effect (23.6%) of wortmannin on LSE-stimulated glucose uptake was demonstrated, suggesting LSE action on glucose transporter translocation. LSE-induced glucose uptake was completely reversed by cycloheximide. In addition, an increased amount of total glucose-transporter-1 protein was observed indicating that new protein synthesis is necessary for elevated glucose transport. LSE also enhanced insulin-stimulated glucose transport. These results suggest that LSE action may be mediated primarily via the synthesis of new transporters and involve both insulin-dependent and independent pathways.
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