Academic Journals Database
Disseminating quality controlled scientific knowledge

The use of global transcriptional analysis to reveal the biological and cellular events involved in distinct development phases of Trichophyton rubrum conidial germination

Author(s): Liu Tao | Zhang Qian | Wang Lingling | Yu Lu | Leng Wenchuan | Yang Jian | Chen Lihong | Peng Junping | Ma Li | Dong Jie | Xu Xingye | Xue Ying | Zhu Yafang | Zhang Wenliang | Yang Li | Li Weijun | Sun Lilian | Wan Zhe | Ding Guohui | Yu Fudong | Tu Kang | Qian Ziliang | Li Ruoyu | Shen Yan | Li Yixue | Jin Qi

Journal: BMC Genomics
ISSN 1471-2164

Volume: 8;
Issue: 1;
Start page: 100;
Date: 2007;
Original page

Abstract Background Conidia are considered to be the primary cause of infections by Trichophyton rubrum. Results We have developed a cDNA microarray containing 10250 ESTs to monitor the transcriptional strategy of conidial germination. A total of 1561 genes that had their expression levels specially altered in the process were obtained and hierarchically clustered with respect to their expression profiles. By functional analysis, we provided a global view of an important biological system related to conidial germination, including characterization of the pattern of gene expression at sequential developmental phases, and changes of gene expression profiles corresponding to morphological transitions. We matched the EST sequences to GO terms in the Saccharomyces Genome Database (SGD). A number of homologues of Saccharomyces cerevisiae genes related to signalling pathways and some important cellular processes were found to be involved in T. rubrum germination. These genes and signalling pathways may play roles in distinct steps, such as activating conidial germination, maintenance of isotropic growth, establishment of cell polarity and morphological transitions. Conclusion Our results may provide insights into molecular mechanisms of conidial germination at the cell level, and may enhance our understanding of regulation of gene expression related to the morphological construction of T. rubrum.
Save time & money - Smart Internet Solutions     

Tango Rapperswil
Tango Rapperswil