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The Use of Nuclear Transfer Procedure for Evaluation of Abattoir Derived Bovine Oocyte

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Author(s): Mohamed Sayed Mohamed Nour | M. Nagano | S. Katagiri | Y. Takahashi

Journal: Journal of Animal and Veterinary Advances
ISSN 1680-5593

Volume: 3;
Issue: 12;
Start page: 800;
Date: 2004;
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ABSTRACT
Using nuclear transfer procedure, this study was conducted to investigate the effect of oocyte quality on the in vitro development of bovine embryos. Immature oocytes from slaughterhouse-collected ovaries were classified into good and poor quality based on their morphological appearance. After maturation culture, the good quality group showed a higher percentage of oocytes with first polar body than the poor one. The oocytes with first polar body from both groups were enucleated, activated, and fused with disaggregated donor blastomeres of 32-cell-stage embryos. Enucleation rates of the oocytes with the first polar body were the same for both groups. After nuclear transfer, no significant difference was detected in fusion and cleavage rates between the 2 groups. However, the embryos reconstituted from good quality oocytes showed significantly higher developmental rate to the blastocyst stage and blastocyst cell number than those from the poor ones. These results indicate the necessity of strict selection of oocytes with good morphology as recipient cytoplast for nuclear transfer.

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