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Use of REP- and ERIC-PCR to reveal genetic heterogeneity of Vibrio cholerae from edible ice in Jakarta, Indonesia

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Author(s): Waturangi Diana E | Joanito Ignasius | Yogi Yogiara | Thomas Sabu

Journal: Gut Pathogens
ISSN 1757-4749

Volume: 4;
Issue: 1;
Start page: 2;
Date: 2012;
Original page

Keywords: Vibrio cholerae | ERIC-PCR | REP-PCR | Edible ice

ABSTRACT
Abstract Background Vibrio cholerae is the causative organism of waterborne disease, cholera. V. cholerae has caused many epidemics and pandemics of cholera for many years. In this study, V. cholerae recovered from edible ice were investigated for their genetic diversity using Enterobacterial Repetitive Intergenic Consensus (ERIC) PCR and Repetitive Extragenic Palindromic (REP) PCR. Isolation was done using selective medium and the presumptive isolates were confirmed through biochemical and serological assays. Results Seventy-five isolates of V. cholerae were recovered from ice samples collected from different locations of Jakarta. Specifically, 19 of them were identified as O1 serotype, 16 were Ogawa, 3 isolates were Inaba and the remaining isolates were non-O1. The fingerprinting profiles of V.cholerae isolated from ice samples were very diverse. Conclusion This result showed that the ERIC sequence is more informative and discriminative than REP sequence for analysis of V. cholerae diversity.
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