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Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts

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Author(s): Ribeiro Aldemir Reginato | Nascimento Vladimir Pinheiro do | Cardoso Martha Oliveira | Santos Luciana Ruschel dos | Rocha Silvio Luís da Silveira

Journal: Brazilian Journal of Microbiology
ISSN 1517-8382

Volume: 33;
Issue: 4;
Start page: 339;
Date: 2002;
Original page

Keywords: Immunomagnetic separation | Salmonella | raw broiler parts

ABSTRACT
This study was conducted aiming to compare the conventional microbiological method to detect Salmonella in broiler parts with the Immunomagnetic Separation method (IMS) followed by plate isolation and also the IMS associated with Rappaport-Vassiliadis broth (RV). The IMS was performed following a pre- enrichment step in buffered peptone water. Sixty-one samples (raw broiler parts) were tested and the results showed that the use of the IMS method alone allowed the isolation of Salmonella in 9 of the tested samples, while the association IMS/RV detected the agent in 30 samples. The conventional microbiological method was able to isolate the agent in 25 opportunities. These results allowed to conclude that the IMS/RV association presented an increased sensitivity and permitted a better isolation of Salmonella. The conclusion was that other means of isolation, in particular those which do not interfere with the growth of bead bounded Salmonella, should be searched.
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Tango Jona
Tangokurs Rapperswil-Jona