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Utilization of RAPD marker to analyze natural genetic variation in Calligonum polygonoides L. - A key stone species of Thar desert

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Author(s): Sangeeta Bewal | Satyawada Rama Rao | Sadha Shivam | Ajay Parida | Santosh Kumar Sharma | Arun Kumar

Journal: International Journal of Integrative Biology
ISSN 0973-8363

Volume: 5;
Issue: 3;
Start page: 148;
Date: 2009;
Original page

Keywords: Calligonum polygonoides | Intra-specific variation | Genetic diversity | Genetic relatedness

ABSTRACT
The species Calligonum polygonoides L. of Polygonaceae is one of the most economically important resources of the Indian desert, playing an important role in the lives of local population. A great range of genetic diversity could be seen in diverse populations of this species which are spread all over western Rajasthan. DNA-based molecular markers are playing increasingly important role in the analysis of genetic diversity in wide range of plants. The present study is an attempt to collect, compile and collate information on the existing natural genetic diversity, at intra-specific and inter-populational levels in C. polygonoides. The present report deals with 22 genotypes of C. polygonoides collected from different districts of Rajasthan using random amplified polymorphic DNA (RAPD) marker. In total 100 fragments with 89 polymorphic bands amplified by 10 random primers, with the polymorphic rate of 89.30% were recorded. The dendrogram was constructed based on the matrix of Dice coefficients by unweighted pair-group mean analysis (UPGMA) using the NTSYS-PC version 1.8 Exeter Software, Setauket, NY. From the analysis it is imperative that predominantly obligate out breeding behavior of C. polygonoides, made these diverse accessions to spread and occupy specific geographical niches in the arid regions of Rajasthan.
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