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VALIDATED RP-HPLC METHOD AS A TOOL FOR THE ESTIMATION OF PRULIFLOXACIN IN PHARMACEUTICAL DOSAGE FORMS

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Author(s): A.Vijaya Lakshmi

Journal: International Journal of Pharmaceutical Research and Development
ISSN 0974-9446

Volume: 2;
Issue: 5;
Start page: 5;
Date: 2010;
Original page

Keywords: Prulifloxacin | RP- HPLC | Validation.

ABSTRACT
A simple reverse phase liquid chromatographic method was developed and validated as per the ICH guidelines for the quantitative determination of Prulifloxacin in pharmaceutical dosage forms. The method was simple, precise, specific and accurate. The mobile phase consists of pH 3.2 phosphate buffer, methanol and acetonitrile( 3:1:1 v/v ). The proposed RP-HPLC method utilizes a 5µm Luna C18 phenomenex column (250mm ×4.6mm) at ambient temperature. The eluent was monitored at 273nm and retention time of Prulifloxacin was 3.910min. The linearity was observed from 12.5-75µg/ml with r 2= 1. The limit of detection and limit of quantitation were found to be0.75µg/ml and 1.5µg/ml respectively.
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