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Validation and Applications of Gas Chromatography-Combustion/isotope Ratio Mass Spectrometric Method to Control Misuse of Androgens in Human

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Author(s): Kang Mi Lee | Ho Jun Kim | Eun Sook Je | Hye Hyun Yoo | Oh Seung Kwon | Changbae Jin | Dong Hyun Kim | Jaeick Lee

Journal: Mass Spectrometry Letters
ISSN 2233-4203

Volume: 2;
Issue: 2;
Start page: 33;
Date: 2011;

Keywords: Steroids | Endogenous | Validation | GC-C/IRMS

ABSTRACT
The misuse of anabolic androgenic steroids is of particular concern in sports and society. Thus, it is of great importance todiscriminate endogenous steroids such as testosterone or testosterone prohormones from their chemically identical syntheticcopies. In this study, gas chromatography-combustion/isotope ratio mass spectrometric (GC−C/IRMS) method has been developedand validated for discriminating the origin of anabolic androgenic steroids. The method involves the solid-phase extraction,enzymatic hydrolysis with β-glucuronidase, HPLC-fractionation for the cleanup and analysis by GC−C/IRMS. The difference(Δ13C) of urinary δ13C values between synthetic analogues and endogenous reference compounds (ERC) by GC−C/IRMS wasused to elucidate the origin of steroids, and intra- and inter-day precision, specificity and isotope fractionation were evaluated.The present GC−C/IRMS method combined with HPLC cleanup was accurate and reproducible enough to be successfullyapplied to the test of urine sample from suspected anabolic steroid abusers.
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Tango Jona
Tangokurs Rapperswil-Jona